LOCUS       40924_24668        5661 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pHIS3U DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pHIS3U
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5661)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5661)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5661)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             242..808
                     /pseudo
                     /gene="his3"
                     /label=his3
                     /note="NotI site is created at the middle of this fragment 
                     (487-494)."
     gene            242..808
                     /pseudo
                     /gene="his3"
                     /label=his3
     regulatory      813..1335
                     /gene="ura4"
                     /regulatory_class="promoter"
     CDS             1336..2127
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      2131..2508
                     /gene="ura4"
                     /regulatory_class="terminator"
     primer_bind     complement(2521..2537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2750..3205
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3487..3591
                     /label=AmpR promoter
     CDS             3592..4449
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4623..5211
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5499..5520
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5535..5565
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5573..5589
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5597..5613
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtgttggca gcgatgaaat tattgactcc ttaattcgca tttcttgtat tcctggaaaa
      301 gataagattt tgatgtgtcc accttcgtac ggaatgtata cggtctctgc aaaaatcaac
      361 gatgttgagg ttgttaaggt tctccttgaa ccagatttca atttgaacgt cgatgctatt
      421 tgcgaaacac tttctaaaga tagtgccatc aaagtctttt ttgcttgttc ccctggtaat
      481 ccaacagcgg ccgctcttaa actcgaggat attaagaaga ttttggagca ccctacatgg
      541 aatggaatcg ttgtcgtcga tgaggcctat attgatttct ctgctcccga tatgtctgcc
      601 ttaactcttg tcaatgaata tccaaacctt gctgtttgcc aaaccctttc gaaatctttc
      661 ggtctagcag gaattcggta agttaatcta agcttttaag cattaatatt atttctttac
      721 taactatgat gtttagtatc ggattttgtt tgacaagtaa acctattgct accattatga
      781 actcattaaa ggctccttat aacattagat ctacaaatcc cactggctat atgtatgcat
      841 ttgtgttaaa aaagtttgta tagattattt aatctactca gcattctttc tctaaatagg
      901 aatttgttac ttaatggaga aaaaaatgtt tcgatttacc tagtgtattt gtttgtatac
      961 tcacgtttaa tttcaaacat ccattctatc ttgtgtaatt tttggcatgg tgaaaaagat
     1021 aatcagcctt ataatcttta caaaagtaag aaattctgta aataagcctt aatgcccttg
     1081 ctttaaatta aaatggttct ttttcatgat aatgtttgca ctttgtgaat atattttaga
     1141 tagttctgtg aggtataatt aagatgtttt agagacttat acaattttgt ctttataaat
     1201 tcttaattga ttttaccatc ccagtttaac tatgcttcgt cggcatctct gcacatgtcg
     1261 tgttttctta ccgtattgtc ctaccaagaa cctctttttt gcttggatcg aaattaaagg
     1321 tttaaaagca aagttatgga tgctagagta tttcaaagct attcagctag agctgagggg
     1381 atgaaaaatc ccattgccaa ggaattgttg gctttgatgg aagaaaagca aagcaacttg
     1441 tcagtcgcgg tcgatttgac gaagaaatcc gaaatcttag aattggtaga taaaattgga
     1501 ccctatgtct gtgttatcaa gacacatatt gacgttgtcg aggatttcga ccaggatatg
     1561 gtagaaaaac tggtggcctt aggtaaaaag catcgttttc ttatctttga ggatcgcaaa
     1621 ttcgcagaca ttggaaatac cgtcaagcta caatatgcat ctggtgtgta caaaattgct
     1681 tcttgggctc atatcacaaa ttgccataca gtgccaggcg agggtattat acaaggcctc
     1741 aaagaagttg gtttaccttt gggacgtggt ctcttgcttt tggctgaaat gtcttccaaa
     1801 ggctctttgg ctactggttc ctacacagag aaaaccttag aatggtttga gaagcatacc
     1861 gatttttgct ttggctttat agctggtcgt cgatttccta accttcaaag cgactacata
     1921 actatgtccc ctggtatcgg cttggatgtt aaaggagacg ggctgggaca gcaatatcgt
     1981 actcctgaag aagtgattgt aaactgcggt agcgatatca tcattgttgg tcgtggagtc
     2041 tatggagctg gtcgtaatcc tgttgtcgaa gccaagagat atagagaagc tggttggaag
     2101 gcatatcagc aaagactttc tcagcattaa aaaaagacta atgtaaaatt tttttggttg
     2161 gttattgaaa aagtcgatgc cttgtttgcg tttgttttcc taggcgtttt atgtcagaag
     2221 gcatttagaa ttagtataca agtactcttt ggtaaaattt tatgtagcga ctaaaatatt
     2281 aactattata gataaacacc ttgggaataa aaagtaattt gctatagtaa tttattaaac
     2341 atgctcctac aacattacca caatcttttc tcttggattg acattgaata agaaaagagt
     2401 gaattttttt agacttgtaa tgataactat gtacaaagcc aatgaaagat gtatgtagat
     2461 gaatgtaaaa taccatgtag acaaacaaga taaaaatcac gtttaaacga gctcgaattc
     2521 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     2581 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     2641 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     2701 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     2761 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2821 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2881 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2941 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     3001 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3061 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     3121 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3181 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     3241 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3301 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     3361 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     3421 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3481 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3541 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3601 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3661 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3721 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3781 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     3841 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3901 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3961 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4021 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4081 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4141 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4201 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4261 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4321 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4381 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4441 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4501 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4561 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4621 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4681 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4741 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     4801 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4861 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4921 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4981 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5041 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5101 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5161 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5221 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5281 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5341 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5401 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5461 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     5521 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     5581 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg
     5641 caggtcgact ctagaggatc c
//
